Table 1. Differentially expressed genes in co-cultured stromal cells.
This table shows the top 20 up- and downregulated genes in INOFs and MSCs co-cultured with Hey.A8 ovarian cancer cells compared to INOFs/MSCs co-cultured with normal ovarian surface epithelial cells. Genes are ranked by pooled fold change.
| Rank | Probe_Id | Symbol | Gene Name | Fold Change v IOSE | Fold Change v non-Co-cultured | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| P Value | MSC | INOF | Pooled | P Value | MSC | INOF | Pooled | ||||
| Upregulated Genes | |||||||||||
|
| |||||||||||
| 1 | 4290040 | NPPB | natriuretic peptide B | 5.5E-06 | 71.86 | 9.42 | 28.23 | 1.8E-07 | 258.78 | 38.01 | 109.94 |
| 2 | 2030309 | KISS1 | KiSS-1 metastasis-suppressor | 0.013 | 10.64 | 1.06 | 9.12 | 1.0E-05 | 16.46 | 4.00 | 10.23 |
| 3 | 1110373 | SERPING1 | serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 | 5.2E-06 | 11.15 | 3.60 | 7.90 | 0.003 | 25.53 | 1.70 | 9.89 |
| 4 | 1470148 | PTGIS | prostaglandin I2 (prostacyclin) synthase | 0.007 | 9.68 | 1.20 | 7.16 | 5.0E-05 | 9.45 | 2.57 | 6.14 |
| 5 | 7150762 | C15orf48 | chromosome 15 open reading frame 48 | 0.005 | 15.22 | 1.37 | 7.11 | 0.012 | 14.81 | 1.07 | 9.05 |
| 6 | 1430487 | MGP | matrix Gla protein | 0.008 | 8.36 | 1.16 | 5.83 | n.s. | 10.59 | 0.39 | 3.73 |
| 7 | 6020468 | MGP | matrix Gla protein | 0.006 | 7.59 | 1.19 | 5.46 | 2.6E-04 | 5.97 | 1.83 | 4.17 |
| 8 | 3460743 | PPP1R14A | protein phosphatase 1, regulatory (inhibitor) subunit 14A | 3.4E-04 | 7.73 | 1.90 | 4.87 | 1.5E-05 | 3.77 | 2.04 | 3.17 |
| 9 | 2940291 | MEGF6 | multiple EGF-like-domains 6 | 0.013 | 5.16 | 1.06 | 4.59 | 8.5E-05 | 4.06 | 1.76 | 2.68 |
| 10 | 940735 | CCDC81 | coiled-coil domain containing 81 | 7.3E-05 | 6.31 | 2.10 | 4.49 | 0.083 | 10.74 | 0.60 | 4.82 |
| 11 | 130044 | ACTG2 | actin, gamma 2, smooth muscle | 0.001 | 6.19 | 1.61 | 4.42 | 8.9E-07 | 7.55 | 3.65 | 4.82 |
| 12 | 6620292 | TINAGL1 | tubulointerstitial nephritis antigen-like 1 | 0.018 | 5.24 | 0.99 | 4.34 | 2.2E-07 | 4.00 | 2.59 | 3.30 |
| 13 | 3710154 | CYP26B1 | cytochrome P450, family 26, subfamily B, polypeptide 1 | 0.015 | 5.32 | 1.03 | 3.81 | 5.8E-04 | 12.57 | 2.00 | 3.72 |
| 14 | 60470 | TIMP3 | TIMP metallopeptidase inhibitor 3 | 0.008 | 3.67 | 1.11 | 3.63 | 4.6E-06 | 3.45 | 2.12 | 2.69 |
| 15 | 6100717 | COL11A1 | collagen, type XI, alpha 1 | 2.8E-05 | 4.34 | 2.07 | 3.49 | 4.4E-06 | 5.26 | 2.46 | 4.27 |
| 16 | 10543 | DHRS3 | dehydrogenase/reductase (SDR family) member 3 | 4.6E-09 | 3.17 | 3.40 | 3.28 | 4.9E-08 | 2.20 | 2.14 | 2.17 |
| 17 | 2450110 | CRYAB | crystallin, alpha B | 2.2E-04 | 3.38 | 1.51 | 3.27 | 1.1E-08 | 2.35 | 2.46 | 2.41 |
| 18 | 4610431 | EPDR1 | ependymin related protein 1 | 2.4E-08 | 3.35 | 2.73 | 3.25 | 0.001 | 8.58 | 1.70 | 5.46 |
| 19 | 4590008 | CRISPLD2 | cysteine-rich secretory protein LCCL domain containing 2 | 1.4E-08 | 3.00 | 3.77 | 3.22 | 1.0E-04 | 3.58 | 1.72 | 2.59 |
| 20 | 4180079 | CNN1 | calponin 1, basic, smooth muscle | 2.6E-05 | 4.00 | 1.95 | 3.20 | 2.9E-07 | 3.51 | 6.68 | 4.16 |
|
| |||||||||||
| Downregulated Genes | |||||||||||
|
| |||||||||||
| 1 | 3360224 | MMP1 | matrix metallopeptidase 1 | 0.024 | 0.18 | 1.08 | 0.22 | 0.012 | 0.33 | 0.94 | 0.39 |
| 2 | 1240440 | TXNIP | thioredoxin interacting protein | 4.9E-10 | 0.22 | 0.25 | 0.22 | 6.2E-06 | 0.42 | 0.21 | 0.37 |
| 3 | 6370221 | DIRAS3 | DIRAS family, GTP-binding RAS-like 3 | 0.011 | 0.18 | 0.91 | 0.23 | 0.001 | 0.70 | 0.65 | 0.68 |
| 4 | 630315 | DHRS9 | dehydrogenase/reductase (SDR family) member 9 | 0.029 | 0.19 | 1.07 | 0.29 | 0.030 | 0.43 | 1.01 | 0.56 |
| 5 | 840204 | HAS2 | hyaluronan synthase 2 | 0.016 | 0.20 | 0.98 | 0.33 | n.s. | 0.23 | 2.16 | 0.41 |
| 6 | 7050575 | PPAP2B | phosphatidic acid phosphatase type 2B | 0.003 | 0.33 | 0.83 | 0.35 | 0.001 | 0.16 | 0.66 | 0.17 |
| 7 | 5810047 | F2RL1 | coagulation factor II (thrombin) receptor-like 1 | 0.025 | 0.11 | 1.10 | 0.35 | 0.069 | 0.21 | 1.28 | 0.60 |
| 8 | 6220019 | PENK | proenkephalin | 0.014 | 0.27 | 0.98 | 0.38 | 0.016 | 0.17 | 0.93 | 0.26 |
| 9 | 6290142 | LOC100008589 | 28S ribosomal RNA | 0.015 | 0.27 | 0.72 | 0.39 | n.s. | 0.84 | 0.58 | 0.69 |
| 10 | 7210681 | LIPG | lipase, endothelial | 2.1E-04 | 0.16 | 0.50 | 0.40 | 2.3E-05 | 0.45 | 0.34 | 0.35 |
| 11 | 2100767 | COLEC12 | collectin sub-family member 12 | 0.019 | 0.38 | 0.97 | 0.41 | 0.008 | 0.26 | 0.91 | 0.28 |
| 12 | 1050068 | F2RL1 | coagulation factor II (thrombin) receptor-like 1 | 0.029 | 0.11 | 1.13 | 0.41 | 0.040 | 0.17 | 1.19 | 0.58 |
| 13 | 1300470 | MAMDC2 | MAM domain containing 2 | 0.002 | 0.81 | 0.39 | 0.42 | 4.2E-05 | 0.59 | 0.49 | 0.50 |
| 14 | 2120452 | MLPH | melanophilin | 0.004 | 0.39 | 0.87 | 0.42 | 1.5E-04 | 0.44 | 0.72 | 0.46 |
| 15 | 770730 | ANXA2 | annexin A2 | 1.8E-04 | 0.28 | 0.55 | 0.43 | 5.9E-04 | 0.19 | 0.58 | 0.36 |
| 16 | 4260017 | CDK5RAP2 | CDK5 regulatory subunit associated protein 2 | 0.006 | 0.29 | 0.88 | 0.43 | 8.7E-04 | 0.31 | 0.72 | 0.43 |
| 17 | 6220097 | PPAP2B | phosphatidic acid phosphatase type 2B | 0.022 | 0.39 | 1.01 | 0.44 | 0.009 | 0.20 | 0.89 | 0.24 |
| 18 | 6980411 | LOC100134259 | uncharacterised miscRNA | 0.004 | 0.37 | 0.87 | 0.44 | 3.3E-04 | 0.44 | 0.71 | 0.49 |
| 19 | 870630 | LYPD1 | LY6/PLAUR domain containing 1 | 2.6E-05 | 0.32 | 0.54 | 0.45 | 1.3E-05 | 0.37 | 0.60 | 0.49 |
| 20 | 6290341 | EFEMP1 | EGF containing fibulin-like extracellular matrix protein 1 | 0.002 | 0.54 | 0.43 | 0.46 | n.s. | 1.65 | 0.67 | 1.02 |