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. Author manuscript; available in PMC: 2016 Mar 15.
Published in final edited form as: Int J Cancer. 2014 Aug 14;136(6):1475–1481. doi: 10.1002/ijc.29123

Figure 3. Inhibition of NT signaling affects NET cell growth.

Figure 3

(A) Equal numbers of BON (left) and QGP-1 (right) cells transfected with siRNA against non-targeting control or NT were plated in 24 well plates. One day after seeding, serum free media were changed and the cell numbers were counted after an additional 48 h incubation (*, P < 0.05 vs. control siRNA). (B) Western blot analysis showing expression of Cyclin D1 and NT in NET cells transfected with control or NT siRNA; β-actin was used as a loading control. (C) The number of colonies compared with the control siRNA in soft agar assay. Colony formation of representative control or NT knockdown QGP-1 cells was assessed over a period of 4 weeks (*, P < 0.05 vs. control siRNA). The colonies were stained with crystal violet solution and quantified using AlphaEaseFC™ software (Alpha Innotech Corporation, San Leandro, CA). (D) BON cells were treated with varying concentrations of SR-48692 (0-20 μM) for 48 and 96 h and cell numbers were counted using a cell counter (*, P < 0.05 vs. vehicle for 48 h; †, P < 0.05 vs. vehicle for 96 h). (E) Western blot analysis representing expression of c-Myc and Cyclin D1 regulated by SR-48692 treatment in BON cells. β-actin was used as a loading control. (F) The number of colonies compared with vehicle in soft agar assay. Colony formation of BON cell treated with vehicle or SR-48692 was assessed over a period of 4 weeks. (*, P < 0.05 vs. vehicle)