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. Author manuscript; available in PMC: 2016 Mar 15.
Published in final edited form as: Int J Cancer. 2014 Aug 5;136(6):1321–1332. doi: 10.1002/ijc.29094

Figure 6.

Figure 6

Disruption of Runx2-Smad interaction (HTY) and deletion of the Runx2 C-terminus (ΔC) result in altered expression of metastasis-related genes. Adenovirus infected cancer cells expressing Runx2 protein showing changes in molecular markers of osteolytic genes was validated in earlier in vitro studies.11 (A) The relative expression of Runx2 target genes (IL-11, PTHrP, OPG (osteoprotegrin), PAI-1, VEGF, and FN1) in response to Runx2 WT and mutant protein overexpression is shown. All data were normalized to GAPDH. Values are mean ± SEM of n=3 experiments analyzed in duplicate. ANOVA analysis was done by comparing the values of all four groups. Statistical results are shown in Table S2. (B) The relationship between changes in gene expression and tumor properties in the Runx2 mutant groups compared to Runx2-WT. Arrows indicate up- and down-regulation of the six most highly expressed genes. BR=bone resorption; TG=tumor growth.