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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Biochim Biophys Acta. 2014 Nov 13;1853(2):370–376. doi: 10.1016/j.bbamcr.2014.11.003

Figure 3. The expression of HDAC4 is dramatically decreased in the growth plate of CAMKK6 mice.

Figure 3

(A) Immunofluorescent histochemistry shows that in WT mice, HDAC4 is highly expressed in the pre-hypertrophic zone (red), but the expression is reduced in the hypertrophic zone. In contrast, CAMKK6 homozygote mice (CAMKK6-TG) exhibit a weaker HDAC4 staining in the pre-hypertrophic zone in comparison to the strong signal in WT mice. Magnified view of the pre-hypertrophic and hypertrophic chondrocytes from WT and CAMKK6-TG mice correspond to the boxed areas. Blue: Nucleus stained by DAPI. Red: HDAC4 stained by Rhoda mine. Purple: overlap of blue and red. Multiple animals were examined and consistent results were obtained (N=4). Safranin-O staining was used to show PG staining on the growth plate. (B) Intensity of the HDAC4 signal was quantified. CAMKK6-TG mice had much lower signal than WT controls. * p<0.05.