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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Invest Dermatol. 2014 Aug 22;135(2):599–608. doi: 10.1038/jid.2014.367

Figure 4. In SS cell lines, inhibition of p38 with small molecules induces apoptosis and BIRB796 works through a p38 mechanism.

Figure 4

(a) H9 and Hut78 cells were treated with increasing concentrations of either SB202, SB203, or BIRB. Apoptosis was measured by flow cytometry, N=3. (b,c) H9 and Hut78 cells were cultured with either Enz+ARA plus BIRB or increasing concentrations of BIRB alone for 5 days. (b–d) Apoptosis was measured by Annexin V staining, N=3. Total cell death is represented as a fold change normalized to DMSO, in the absence of BIRB treatment. Comparisons were made between those cells with treated with BIRB alone and cells treated with BIRB in the presence of Enz+ARA. (b) H9 (c) Hut78 (d) Raw cell death (Annexin V positive cells), no BIRB treatment.