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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Liver Int. 2014 Jul 28;35(2):532–541. doi: 10.1111/liv.12633

Figure 5. Decreased liver miR-122 is associated with transcriptional repression and concomitant increase in the serum.

Figure 5

Liver and serum was collected from mice fed with either MCD or MCS diet for 1, 2, 3, 6 or 8 weeks. Primary miR-122 (pri-miR-122) expression was measured in the livers and hepatocytes of MCS or MCD diet-fed mice (n=6–8) using TaqMan primer assay, normalized to GAPDH [A]. HNF6 mRNA was quantified [B]. Total RNA was isolated from 50ul serum samples and equal volume of RNA was used to measure miR-122 levels using TaqMan miRNA assay. Spiked synthetic Cel-miR-39 was used for normalization [C]. Serum from 6 week MCS or MCD fed mice was fractionated into exosome and protein-rich fractions using ExoQuick method and miR-122 expression was determined using TaqMan miRNA assay [D]. [*] indicates p<0.05 vs. MCS.