Table 3.

Larvicidal activity of crude protein from water extract of Gossypium hirsutum (BollgaurdII) against II ^nd, III^rd and IV^th instar larvae of Aedes aegypti and Anopheles stephensi

Mosquito species	Larval stage (Instars)	LC50 ±SE (mg lit−1)	95% Fiducial limits (LCL-UCL)	LC90±SE (mg lit−1)	95% Fiducial limits (LCL-UCL)	Regression equation
Aedes aegypti	II	105.72±25.84	35.50–147.30	273.28± 25.56	232.00–345.43	Y=4.53+0.0127X
	III	126.19±25.65	56.87–168.45	314.17±27.89	269.46–391.62	Y=3.73+0.0140X
	IV	137.88±17.59	96.13–169.37	350.23± 30.23	302.8–432.062	Y=2.29+0.0195X
Anopheles stephensi	II	138.23±23.18	78.18–177.71	317.83±27.09	274.38–392.16	Y=3.30+0.0150X
	III	134.04±04	70.56–174.23	315.33±27.05	95.71–389.51	Y=3.30+0.0150X
	IV	154.25± 16.98	115.178–185.6	371.42±32.6	320.54–460.49	Y=1.92+0.0197X

Mortality rate (significant at P< 0.05 level), X concentration (significant at P< 0.05 level), LC₅₀ lethal concentration that kills 50% of the exposed larvae, LC₉₀ lethal concentration that kills 90% of the exposed larvae, SE standard error (all values are mean of four replicates), LCL lower confidence limit, UCL upper confidence limit