Fig. 5.
A PERK inhibitor reduces HCC burden in the orthotopic mouse model. a Immunoblotting for phospho-Perk, Atf4 and phospho-eIf2α in the lysates of isolated tumors after 25 weeks of DEN followed by treatment with a PERK inhibitor or vehicle. Experiments were repeated twice with similar results. b Reticulin staining to quantify the tumor burden as shown by mean total tumor surface. Arrows indicate tumors. c 18F-Choline positron emission tomography visualizes cell membrane synthesis after the indicated treatments. Arrows indicate the left kidney for reference density. Standardized uptake values of the mouse livers are presented as the mean ± SD. One-way ANOVA was applied for statistical analysis. *p < 0.05, **p < 0.01