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. 2014 Dec 22;47(1):75. doi: 10.1186/0717-6287-47-75

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© Yin et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Schematic of the simultaneous BrdU and WST-1 measurements. The MRP-14 protein was added at concentrations of 1, 10, 50, 100, 200, 400 and 800 ng/ml. Before adding the WST-1 reagent into the plate at the time point of 24 h, the BrdU was added and incubated for 2 h. After the WST-1 measurement was conducted, the BrdU assay was performed.