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. Author manuscript; available in PMC: 2015 Jan 11.
Published in final edited form as: J Med Chem. 2010 Jul 22;53(14):5179–5185. doi: 10.1021/jm100139b

Table 5.

Binding Affinities of Fluorescently Tagged Tracers to WDR5 Protein

Ac-amino acid sequence-linker-K(5-FAM)-NH2
tracer name amino acid sequence linker Kd ± SD (µM)
WIN–FAM-1 GSARAEVHLRKS βA-βA-βAa 0.86 ± 0.15
WIN–FAM-2 GSARAEVHLRKS Ahx-Ahxb 0.94 ± 0.15
10mer-Ala-FAM ARAEVHLRKS Ahx-Ahx 0.014 ± 0.002
10mer-Thr-FAM ARTEVHLRKS Ahx-Ahx 0.001 ± 0.0003
a

βA = betaalanine.

b

Ahx = 6-amino hexanoic acid.