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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Biochem Pharmacol. 2014 Nov 20;93(2):163–170. doi: 10.1016/j.bcp.2014.11.005

Figure 2. Lipid raft disruption does not directly affect CLEC-2-mediated platelet aggregation.

Figure 2

Washed human platelets were incubated with 5mM MβCD and 200 μg/ml cholesterol (control) for 1 hour or GF109203X (5μM), a pan-PKC inhibitor for 5 min or MβCD (10mM) for 1 hour prior to stimulation with rhodocytin (100nM), human CLEC-2 Ab (10μg/ml) and fucoidan (50μg/ml) along with or without 2MesADP (100nM) for 3 minute at 37°C under stirring conditions. All platelets were incubated with IV.3 F(ab’)2 fragments (10μg/ml) for 5 min to block FcγRIIA receptor. Aggregation tracings are representation of 3 independent experiments.