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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Biochem Pharmacol. 2014 Nov 20;93(2):163–170. doi: 10.1016/j.bcp.2014.11.005

Figure 4. CLEC-2 receptor signaling in methyl-β-cyclodextrin (MβCD) treated and untreated platelets.

Figure 4

Washed human platelets treated with or without MβCD (5mM) was activated with rhodocytin (100nM), human CLEC-2 Ab (10μg/ml) and fucoidan (50μg/ml) for 1 min and samples were analyzed using SDS-PAGE/Western blotting and probed with a phospho-Syk and phospho-PLCγ2 antibody. Data are representation of 3 independent experiments. Graphs represent mean ± S.E. of % aggregation from at least three different experiments (NS, P≥0.05).