Table 1.
Gene | Forward primer | Reverse primer | Amplicon length, bp |
---|---|---|---|
mus TNF-α | TATGGCTCAGGGTCCAACTC | CTCCCTTTGCAGAACTCAGG | 174 |
mus IL-6 | ACGGCCTTCCCTACTTCACA | CATTTCCACGATTTCCCAGA | 129 |
mus IL-1α | GCAACGGGAAGATTCTGAAG | TGACAAACTTCTGCCTGACG | 177 |
Δmus Actb | CGTGCGTGACATCAAAGAGAA | TGGATGCCACAGGATTCCAT | 201 |
mus IFN-γ | ACTGGCAAAAGGATGGTGAC | GCTGATGGCCTGATTGTCTT | 98 |
mus IL12a | ATGACCCTGTGCCTTGGTAG | GAAGCAGGATGCAGAGCTTC | 241 |
mus IL12b | AAGGAACAGTGGGTGTCCAG | GGCAAACCAGGAGATGGTTA | 196 |
Reference gene