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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cancer Prev Res (Phila). 2014 Nov 3;8(1):27–36. doi: 10.1158/1940-6207.CAPR-14-0193

Figure 3. β-adrenoceptor expression in JB6 P+ cells.

Figure 3

(A) RT-PCR analysis revealed that JB6 P+ cells only express β2-ARs. The positive control (+Ctr) is Mouse Universal Reference cDNA. (B) Binding assays utilizing 3H-CGP and specific β-blockers: nebivolol (β1-AR), ICI-118,551 (β2-AR), and L-748,337 (β3-AR) indicate that only inhibition of the β2-AR showed significant (p < 0.05) displacement of CGP in a dose-dependent manner via an ANOVA with Tukey-Kramer post hoc test, indicated by an asterisk (*); n ≥ 6. (C) Functional assays examining cAMP accumulation utilizing specific agonists: xamoterol (β1-AR), formoterol (β2-AR), or L-755,507 (β3-AR) and carvedilol demonstrate that only stimulation of the β2-AR resulted in a statistically significant increase in cAMP compared to control (p < 0.05) via Kruskal-Wallis multiple-comparison test, indicated by an asterisk (*), which was reversed by carvedilol treatment.