Table 2.
Genes differentially expressed in ethanol classified into biological groups based on GO terms by DAVID analysis
| Description | Genes | % | p-value |
|---|---|---|---|
| Oxidation-reduction activity |
acdh-1 ↑, acdh-9 ↓, alh-1 ↑, alh-2 ↑, alh-5 ↑, cyp-25A2 ↑, cyp-32B1 ↓, cyp-33C6 ↓, cyp-34A4 ↓, cyp-34A6 ↑, cyp-37B1 ↓, ddo-1 ↓, ddo-2 ↑, dhs-19 ↑, dhs-27 ↑, dhs-30 ↑, dpyd-1 ↓, fat-5 ↑, fmo-2 ↑, gpdh-2 ↑, hgo-1 ↓, idh-1 ↑, msra-1 ↑, pah-1 ↓, sod-3 ↑, sodh-1 ↑, tyr-3 ↑, B0513.5 ↓, C06A8.1 ↑, C07D8.6 ↓, C11E4.2 ↓, C28H8.11 ↓, F35C8.5 ↓, F55B11.1 ↓, R04B5.5 ↑, Y38F1A.6 ↑ |
6.1 | 2.01×10−9 |
| Proteolysis |
bath-43 ↓, cpr-2 ↓, cpr-3 ↑, cpr-4 ↑, cpt-3 ↑, cpt-5 ↑, dpf-6 ↑, mfb-1 ↓, nas-24 ↓, skr-3 ↑, skr-4 ↑, ubc-3 ↓, ubc-8 ↓, wrt-1 ↑, C17B7.10 ↓, F32H5.1 ↓, F53A9.1 ↓, F53B1.6 ↑, K12H4.7 ↓, R05H10.7 ↓, T22F3.2 ↓, Y16B4A.2 ↑ |
3.8 | 0.1 |
| Determination of lifespan |
acdh-1 ↑, aqp-1 ↑, asah-1 ↓, dao-3 ↑, ftn-1 ↓, gei-7 ↑, hsp-12.6 ↓, inf-1 ↑, mtl-2 ↓, mup-4 ↑, pah-1 ↓, sod-3 ↑, sodh-1 ↑ |
2.2 | 0.06 |
| Nitrogen compound biosynthetic process |
dpyd-1 ↓, gcy-11 ↓, odc-1 ↑, smd-1 ↑, B0513.5 ↓, C02E7.1 ↓, C06G3.5 ↓, F38B6.4 ↑, M153.1 ↑, M02D8.4 ↑, R03D7.1 ↑, R08E5.2 ↓, Y62E10A.13 ↑ |
2.2 | 0.04 |
| Cofactor metabolic process |
dao-3 ↑, dhs-19 ↑, gei-7 ↑, hacd-1 ↑, pnk-1 ↑, C44B7.10 ↑, R03D7.1 ↑, Y106G6E.4 ↓, ZK1320.9 ↑ |
1.5 | 0.04 |
| Carboxylic acid biosynthetic process |
fat-5 ↑, B0513.5 ↓, F35C8.5 ↓, M02D8.4 ↑, M153.1 ↑, R03D7.1 ↑, R08E5.2 ↓, Y62E10A.13 ↑ |
1.4 | 0.002 |
| Cellular amino acid catabolic process |
hgo-1 ↓, pah-1 ↓, B0513.5 ↓, C28H8.11 ↓, F47B10.2 ↓, Y51H4A.7 ↓ | 1.0 | 0.0009 |
| Lipid modification | paqr-1 ↓, ugt-12 ↑, ugt-17 ↑, ugt-20 ↑, ugt-25 ↑, ugt-31 ↑ | 1.0 | 0.03 |
| Organophosphate metabolic process |
asm-2 ↓, asm-3 ↓, gpdh-2 ↑, C07E3.9 ↓, C55B6.1 ↓, M153.1 ↑ | 1.0 | 0.08 |
| Fatty acid metabolic process |
acs-2 ↑, fat-5 ↑, hacd-1 ↑, paqr-1 ↓, F35C8.5 ↓ | 0.9 | 0.05 |
| Lipid catabolic processes |
asm-2 ↓, asm-3 ↓, C07E3.9 ↓, F09B12.3 ↓, Y73B6BL.4 ↓ | 0.9 | 0.06 |
| Glycerol metabolic process |
gpdh-2 ↑, C55B6.1 ↓, K10B3.6 ↓, M153.1 ↑ | 0.7 | 0.009 |
| Folic acid and derivative biosynthetic process |
dao-3 ↑, metr-1 ↑, Y106G6E.4 ↓ | 0.5 | 0.02 |
Differentially expressed genes from the combined set of experiments (Figure 1) were analyzed by DAVID (25,26). Down arrows denote downregulated genes. Up arrows denote upregulated genes. Percentages correspond to the number of genes from each category out of the total number differentially expressed.