Table 3.
Top 30 genes up- and downregulated by ethanol based on the fold change
| Upregulated Gene |
FPKMcontrol | FPKMethanol | Fold Change |
q-value | Function | Human homolog |
|---|---|---|---|---|---|---|
| F46C5.1 | 0.91 | 242.56 | 265.18 | 0.004 | Unknown | Unknown |
| D1086.9 | 0.48 | 120.50 | 248.64 | 0.004 | Unknown | Filaggrin |
| H39E23.3 | 0.23 | 58.10 | 242.86 | 0.004 | Unknown | Neurofilament heavy polypeptide |
| F28F8.2 (acs-2) |
2.35 | 511.64 | 217.34 | 0.004 | Acyl-CoA synthetase | Long-chain-fatty-acid- CoA ligase |
| F15A4.8 (chil-28) |
0.22 | 30.48 | 138.55 | 0.004 | Chitinase-like | Chitotriosidase-1 |
| Y69H2.14 | 7.21 | 912.42 | 126.38 | 0.004 | Unknown | Collagen alpha-1 (II) chain |
| K12G11.3 (sodh-1) |
7.04 | 726.39 | 103.12 | 0.004 | Sorbitol dehydrogenase | Alcohol dehydrogenase 4 |
| T22F3.11 | 2.44 | 248.25 | 101.57 | 0.004 | Unknown | Solute carrier family 17 |
| C55B7.4 (acdh-1) |
2.45 | 242.64 | 98.78 | 0.004 | Short-chain acyl-CoA dehydrogenase |
Short/branched chain specific acyl-CoA dehydrogenase |
| F56D6.9 | 4.00 | 383.84 | 95.94 | 0.004 | Unknown | Unknown |
| T09F5.9 (clec-47) |
0.77 | 68.98 | 88.61 | 0.004 | C-type lectin | Lymphocyte antigen 75 |
| K08C7.5 (fmo-2) |
0.82 | 69.07 | 83.84 | 0.004 | Flavin-containing monooxygenase |
Dimethylaniline monooxygenase |
| F46F2.3 | 3.83 | 312.80 | 81.63 | 0.004 | Unknown | Heterogeneous nuclear ribonucleoprotein U-like protein 1 |
| K04F1.15 (alh-2) |
0.08 | 5.12 | 63.51 | 0.045 | Aldehyde dehydrogenase |
Aldehyde dehydrogenase |
| C50F7.2 (clx-1) |
0.24 | 15.38 | 62.62 | 0.004 | Collagen sequence X- hybridizing |
Collagen alpha-1 (III) chain |
| R09B5.6 (hacd-1) |
5.10 | 317.95 | 62.25 | 0.004 | Hydroxy-acyl-CoA dehydrogenase |
Hydroxy-acyl-CoA dehydrogenase |
| Y68A4A.13 | 1.48 | 87.96 | 59.39 | 0.004 | Unknown | Unknown |
| ZK355.8 | 2.76 | 157.07 | 56.76 | 0.004 | Unknown | Unknown |
| Y39B6A.27 | 0.46 | 25.60 | 54.79 | 0.004 | Unknown | UNC93-like protein MFSD11 |
| ZK355.3 | 0.69 | 37.32 | 54.01 | 0.004 | Unknown | Unknown |
| T27E7.6 | 0.11 | 5.88 | 50.25 | 0.017 | Unknown | Golgin subfamily B member 1 |
| T10H9.5 (pmp-5) |
1.23 | 61.79 | 50.18 | 0.004 | Peroxisomal membrane protein related |
ATP-binding cassette sub-family D member 4 |
| B0507.8 | 1.23 | 61.81 | 50.01 | 0.004 | Unknown | Cingulin-like protein 1 |
| B0507.10 | 1.77 | 85.64 | 48.36 | 0.004 | Unknown | Uncharacterized protein |
| K08D8.3 | 0.28 | 13.39 | 47.12 | 0.004 | Unknown | Unknown |
| W06D12.3 (fat-5) |
1.65 | 69.16 | 41.86 | 0.004 | Delta-9 fatty acid desaturase |
Acyl-CoA desaturase |
| C35C5.8 | 1.11 | 44.79 | 40.23 | 0.004 | Unknown | Unknown |
| Y2H9A.6 | 0.28 | 11.33 | 39.83 | 0.012 | Unknown | Unknown |
| F21C10.10 | 19.86 | 783.18 | 39.41 | 0.004 | Unknown | Unknown |
| T22B7.3 | 1.20 | 45.56 | 37.92 | 0.004 | Unknown | Unknown |
| W03G1.7 (asm-3) |
17.78 | 0.15 | −112.43 | 0.004 | Acid sphingomyelinase | Sphingomyelin phosphodiesterase |
| T28A11.5 | 34.00 | 0.95 | −35.77 | 0.004 | Unknown | Unknown |
| K09C4.1 | 6.38 | 0.20 | −31.59 | 0.013 | Unknown | Solute carrier family 2 |
| C17B7.10 | 5.35 | 0.17 | −30.68 | 0.009 | Thermolysin-like zinc metallopeptidase |
Unknown |
| F42E8.1 | 2.87 | 0.10 | −28.68 | 0.044 | Unknown | Unknown |
| F08F3.4 | 97.94 | 3.47 | −28.16 | 0.004 | Unknown | Inactive L-threonine 3- dehydrogenase |
| ZK84.7 (ins-20) |
356.31 | 13.47 | −26.44 | 0.004 | Type-alpha insulin like molecule |
Unknown |
| C17B7.4 | 6.81 | 0.26 | −25.44 | 0.033 | Unknown | Unknown |
| T08G5.10 (mtl-2) |
1107.96 | 49.51 | −22.37 | 0.004 | Metallothionein | Keratin-associated protein 5-9 |
| Y56A3A.33 | 923.76 | 43.03 | −21.46 | 0.004 | Unknown | Uncharacterized protein |
| W09C3.7 | 12.26 | 0.58 | −20.97 | 0.004 | Unknown | Unknown |
| R09E10.13 | 164.11 | 8.22 | −19.96 | 0.004 | Unknown | Unknown |
| C28C12.5 (spp-8) |
1372.15 | 68.79 | −19.94 | 0.004 | Saposin-like protein | Uncharacterized protein |
| C05E4.5 (str-133) |
2.92 | 0.15 | −18.79 | 0.046 | Seven transmembrane receptor |
Unknown |
| R05H10.7 | 17.89 | 1.00 | −17.88 | 0.007 | Unknown | Unknown |
| Y82E9BR.11 | 6.28 | 0.35 | −17.55 | 0.045 | Unknown | Unknown |
| K05F6.10 | 102.49 | 5.90 | −17.36 | 0.004 | Unknown | Unknown |
| K07H8.6 (vit-6) |
0.61 | 0.03 | −16.89 | 0.017 | Vitellogenin precursor protein |
BMP-binding endothelial regulator protein |
| F15E11.12 (pud-4) |
36.83 | 2.24 | −16.39 | 0.025 | Protein up-regulated in daf-2 loss of function |
Unknown |
| T21C9.9 | 34.50 | 2.19 | −15.74 | 0.004 | Unknown | Unknown |
| T09H2.1 (cyp-34A4) |
70.39 | 4.51 | −15.59 | 0.004 | Cytochrome P450 | Cytochrome P450 2C8 |
| C05B5.6 (fbxa-155) |
12.71 | 0.83 | −15.19 | 0.004 | F-box A protein | Unknown |
| F47B10.2 (haly-1) |
181.89 | 12.18 | −14.93 | 0.004 | Histidine ammonia lyase |
Histidine ammonia lyase |
| F10G7.6 | 3.95 | 0.27 | −14.56 | 0.017 | Unknown | Unknown |
| K03H6.2 | 7.55 | 0.52 | −14.26 | 0.004 | Unknown | Unknown |
| F40E3.5 | 463.72 | 33.25 | −13.94 | 0.004 | Unknown | Serine/threonine-protein phosphatase PP1 |
| F47B8.10 | 41.28 | 2.96 | −13.94 | 0.004 | Glucose-6-phosphate transporter |
Glucose-6-phosphate translocase |
| C52D10.13 (col-138) |
5.97 | 0.42 | −13.93 | 0.037 | Collagen | Uncharacterized protein |
| F36D1.2 (sre-22) |
19.98 | 1.47 | −13.57 | 0.004 | Serpentine receptor, class epsilon |
Unknown |
| F42G4.6 | 4.82 | 0.35 | −13.40 | 0.033 | Nematode-specific sperm protein |
Unknown |
Only genes with detectable transcript levels in both control and ethanol-treated worms are included here. Annotated protein function and human homolog information based on the best BLASTp match were obtained from WormBase. The significant genes listed have q-values ≤ 0.05.