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. 2014 Oct 22;58(4):2449. doi: 10.4081/ejh.2014.2449

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©Copyright S. Grecchi and M. Malatesta

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Transmission electron micrographs of HeLa cells at different times after PKH26 staining; DAB photo-oxidation. a) Immediately after PKH26 treatment a fine granular photo-oxidation product diffusely borders the cell surface (thin arrows). b,c) Thirty min after PKH26 staining, many invaginations of the cell membrane (asterisk) as well as small endocytotic vesicles (arrowheads) just beneath cell surface show the photo-oxidation reaction product. d-f) After one-three h after PKH26 staining, photo-oxidation precipitates are evident in many multivesicular bodies (open arrows) as well as in multilamellar bodies inside autophagic vacuoles (arrows). These vacuolar structures often occur near the cell nucleus (N). Scale bars: 500 nm.