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Primer sets used for amplification of kDNA genes in LAMP assay for L. infantum
| Primer | Type | Length mer | Sequence (5 ′-3′) |
|---|---|---|---|
| FIP(F1C+F2) | Forward Inner primer | 38 | GTGGTCTGGGTAGTGGCTT-GGTT-GGATTGGCCTGAAAC |
| BIP(B1C+B2) | Backward Inner primer | 36 | TGCGCCTTGGACTTTCGG-TAATGTTGGGCACATGCT |
| F3 (FOP) | Forward outer primer | 19 | TGGTTGGAAATTGGCTCTC |
| B3 (BOP) | Backward outer primer | 22 | GATTGAGTTGGATTCATTGACG |
| FLP | Forward Loop primer | 20 | CTGGGTCTGGAGGCTTTATT |
| BLP | Backward Loop primer | 22 | GGCTTTTGTTGGGATTTTGTTG |
