Figure 7.

Increase of AgRP gene expression in hypothalamus of SA-treated db/db mice. Diabetic db/db mice were orally intubated with SA (10 mg kg⁻¹ body weight/day) or PBS. Eight weeks later, the hypothalamus was removed, and the expression of (a) AgRP, (b) POMC, and (c) MC4R mRNA was analyzed by RT-qPCR and normalized by cyclophilin expression. The fold change was calculated as a ratio of the expression level in PBS-treated diabetic db/db mice. ^* P < 0.05, ^** P < 0.01 compared with PBS-treated db/db mice (n = 5–10/group).