Table 1.
A typical purification scheme of camel liver G6PD.
| Purification steps* | Total protein (mg) | Total activity (unit†) | Specific activity‡ | Yield (%) |
Fold purification |
|---|---|---|---|---|---|
| Crude extract | 156.28 | 4.4801 | 0.0286 | 100 | 1.00 |
| Ammonium sulphate fraction | 49.530 | 3.8517 | 0.0777 | 85.97 | 2.719 |
| DEAE-cellulose | |||||
| Camel liver G6PD (0.1 M NaCl) | 18.032 | 3.0527 | 0.16929 | 68.13 | 5.919 |
| Sephacryl S-300 | |||||
| Camel liver G6PD | 9.7562 | 2.5990 | 0.26647 | 58.01 | 9.317 |
| 2′, 5′ ADP Sepharose 4B | |||||
| Camel liver G6PD | 1.2320 | 2.2230 | 1.80438 | 49.61 | 63.09 |
*All data is based on 10 gm camel liver.
†One unit of glucose-6-phosphate dehydrogenase enzyme activity is identified as the amount of enzyme required to reduce 1 μmol of NADP+ per minute.
‡Specific activity is expressed as units/mg protein.