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. 2014 Dec 24;2014:985174. doi: 10.1155/2014/985174

Figure 5.

Figure 5

The effect of KRGE on RGS5 mRNA expression in LPS-activated RAW264.7 cells and BV-2 microglial cells. RAW264.7 cells were pretreated with KRGE for 30 min and stimulated with LPS for 18 h. RGS5 (a) mRNA expressions were determined by real time PCR. (b) BV2 microglia cells were transfected with scramble siRNA or RGS5 siRNA (100 nM) and then stimulated with LPS for 18 h to determine RGS5 mRNA expressions using RT-PCR (c) and real time PCR ((d) and (e)). The effect of RGS5 siRNA transfection was evaluated on mRNA expression of iNOS, COX-2 and IL-1β ((c) and (d)), and RGS5 itself (e) in KRGE treated cells by RT-PCR and real time PCR, respectively. Each bar graph represented mean ± SEM for 4 independent experiments. * P < 0.05, ** P < 0.02, *** P < 0.001, compared to LPS. # P < 0.05, ## P < 0.005, ### P < 0.001 compared to wild type or scrambled group.