Table 1.
The sequence of oligonucleotides.
| Gene | Primer | Oligonucleotide sequence (5′-3′) |
|---|---|---|
| GAPDH | F | 5′CAATGAATACGGCTACAGCAAC3′ |
| R | 5′AGGGAGATGCTCAGTGTTGG3′ | |
|
| ||
| iNOS | F | 5′CCCTTCCGAAGTTTCTGGCAGCAGC3′ |
| R | 5′GGCTGTCAGAGCCTCGTGGCTTTGG3′ | |
|
| ||
| COX-2 | F | 5′CACTACATCCTGACCCACTT3′ |
| R | 5′ATGCTCCTGCTTGAGTATGT3′ | |
|
| ||
| IL-1β | F | 5′CAGGGTGGGTGTGCCGTCTTTC3′ |
| R | 5′TGCTTCCAAACCTTTGACCTGGGC3′ | |
|
| ||
| TNF-α | F | 5′TTGACCTCAGCGCTGAGTTG3′ |
| R | 5′CCTGTAGCCCACGTCGTAGC3′ | |
|
| ||
| IL-6 | F | 5′GCTGGAGTCACAGAAGGAGTGGC3′ |
| R | 5′GGCATAACGCACTAGGTTTGCCG3′ | |
|
| ||
| Leptin | F | 5′TGAACAAAGGGGCTTGGGTT3′ |
| R | 5′TGTGCCCTGAAATGCGGTAT3′ | |
|
| ||
| Adiponectin | F | 5′GCTACTGTTGCAAGCTCTCCT3′ |
| R | 5′TCGTAGGTGAAGAGAACGGC3′ | |
|
| ||
| RGS5 | F | 5′CCCAAGGAGTGAACCGGCTGT3′ |
| R | 5′GCACTGCCCTTGAGGCACCC3′ | |