[Table/Fig-1]:

Primers used for detection of S. Typhi and S. ParaTyphi A serotypes in clinical specimens., PCR: Polymerase chain reaction

Gene	Primer	Primer sequence (5’-3’)	Products size	Target positions	PCR condition, (number of cycles)	References
Flagellin (fliC) gene
Primary	ST1	ACTGCTAAAACCACTACT	495 bp	1036-1056	94oC, 5 min; 52oC, 1 min; 72oC, 1 min, (35)	[19,20]
	ST2	TTAACGCAGTAAAGACAG		1513-1530
Nested	ST3	AGATGGTACTGGCGTTGCTC	364 bp	1072-1089	94oC, 5 min; 63oC, 1 min; 72oC, 1 min, (35)
	ST4	TGGAGACTTCGGTCGCGTAG		1416-1435
Putative fimbrial (staA) gene
	staA F1	TGGTTACATGACCGGTAGTC	537 bp	33-52	94oC, 1 min; 56oC, 1min; 72oC, 1 min (35)	[21]
	staA R1	TAGCTGCCGCAATGGTTATG		569-550
	staA F2	CATCGGCACGAACGTAAGAC	377 bp	66-85	94oC, 1 min; 63oC, 1min; 72oC, 1min (35)
	staA R2	TCAAGCGACTGATGGTGACG		442-423
Putative fimbrial protein (stkG) gene
Primary	stkG F1	CGTTTACTGAGGTCACAGGCATC	427 bp	96-118	94oC, 5 min; 57oC, 1 min; 72oC, 1 min, (35)	This study
	stkG R1	CACATTGTTCTCGGAGACCCCA		522-501
Nested	stkG F2	CAATGGCTTCTGGCGAACTGTC	299 bp	138-159	94oC, 5 min; 61oC, 1 min; 72oC, 1 min, (35)
	stkG R2	GTGGAGAAAGATCAGACCACCGAG		366-343