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. 2014 Nov 20;8(11):PC01–PC04. doi: 10.7860/JCDR/2014/8970.5104

[Table/Fig-1]:

Primers used for identification of Streptococcus pneumoniae serotype

Primer Sequences Accession number (GenBank) Amp-licon size (bp)
S.pne4-F S.pne4-R 5’-CTGTTACTTGTTCTGGACTCTCGATAATTGG-3’ 5’-GCCCACTCCTGTTAAAATCCTACCCGCATTG-3’ AF316639 430
S.pne6B-F S.pne6B-R 5’-CGACGTAACAAAGAACTAGGTGCTGAAAC-3’ 5’-AAGTATATAACCACGCTGTAAAACTCTGAC-3’ AF316640 220
S.pne9V-F S.pne9V-R 5’-CTTCGTTAGTTAAAATTCTAAATTTTTCTAAG-3’ 5’-GTCCCAATACCAGTCCTTGCAACACAAG-3’ AF402095 753
S.pne14-F S.pne14-R 5’-GAAATGTTACTTGGCGCAGGTGTCAGAATT-3’ 5’-GCCAATACTTCTTAGTCTCTCAGATGAAT-3’ X85787 189
S.pne18C-F S.pne18C-R 5’-CTTAATAGCTCTCATTATTCTTTTTTTAAGCC -3’ 5’-TTATCTGTAAACCATATCAGCATCTGAAAC-3’ AF316642 573
S.pne19F-F S.pne19F-R 5’-GTTAAGATTGCTGATCGATTAATTGATATCC-3’ 5’-GTAATATGTCTTTAGGGCGTTTATGGCGATAG-3’ U09239 304
S.pne23F-F S.pne23F-R 5’-GTAACAGTTGCTGTAGAGGGAATTGGCTTTTC-3’ 5’-CACAACACCTAACACTCGATGGCTATATGATTC-3’ AF057294 384