Table 1.
Crested ibis | Little egret | |||
---|---|---|---|---|
De novo sequence | ||||
Insert size (bp) | Total data (Gb) | Sequence coverage ( x genome) | Total data (Gb) | Sequence coverage (x genome) |
170-800 | 141 | 113 | 53 | 42 |
2-20 × 103 | 54 | 43 | 36 | 29 |
Total | 195 | 156 | 89 | 71 |
Assembly | ||||
N50 (kb) | Total size (Gb) | N50 (kb) | Total size (Gb) | |
Contigs | 67 | 1.24 | 24 | 1.16 |
Scaffolds | 10,687 | 1.26 | 3,051 | 1.21 |
Annotation | ||||
Number | Total length (Mb) | Number | Total length (Mb) | |
Repeats | 840,220 | 80 | 861,413 | 86 |
Genes | 17,179 | 343 | 17,032 | 326 |
Coding exons | 148,003 | 24 | 139,080 | 23 |
Resequencinga | ||||
Total data (Gb) | Sequence coverage (x genome) | Total data (Gb) | Sequence coverage (x genome) | |
Each individual | 25 | 20 | 30 | 24 |
All individuals | 200 | 160 | 149 | 119 |
SNP calling | ||||
Total SNPs | Mutation rateb (θS) (×10-3) | Total SNPs | Mutation rateb (θS) (×10-3) | |
Autosome | 1,188,930 | 0.33 | 8,876,153 | 2.91 |
Coding exons | 13,459 | 0.17 | 91,820 | 1.45 |
aPaired-end libraries with insert size of 500 bp were used for resequencing. Samples were from eight crested ibis and five little egrets.
bThe population mutation parameter θS = K/aL, a = 1 + 1/2 + … + 1/(n-1), where K is the number of variant sites found by sequencing n chromosomes in a region of length L.