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. 2014 Dec 29;5(6):144. doi: 10.1186/scrt534

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© Boerckel et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. The licensee has exclusive rights to distribute this article, in any medium, for 12 months following its publication. After this time, the article is available under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Principal components of a microcomputed tomography scanner. A micro-focus x-ray tube, or synchrotron emitter for monochromatic beam generation, produces radiation, which is collimated and passed through the object. The radiation is attenuated by the sample, and this attenuation is measured by a charge-coupled device (CCD) camera with a phospholayer coating to convert x-rays to visible light. A three-dimensional rendering of the sample is achieved by scanning at different angles of rotation and reconstructing through transformation of two-dimensional projections.