Table 1.
Parameter | L2/3 neurons (n = 15) | L4 neurons (n = 18) | L5A neurons (n = 8) | L2/3 vs L4 | L4 vs L5A | L2/3 vs L5A |
---|---|---|---|---|---|---|
Vr (mV) | −78 ± 1 | −72 ± 1 | −69 ± 2 | *0.00079 | 0.089 | *0.00095 |
Rinput (Mohm) | 65 ± 4 | 71 ± 7 | 148 ± 17 | 0.99 | *0.00095 | *0.00034 |
Cm (pF) | 146 ± 12 | 109 ± 11 | 90 ± 7 | 0.038 | 0.42 | *0.0061 |
Sag (%) | 2.8 ± 0.3 | 4.3 ± 0.6 | 6.0 ± 1.0 | 0.057 | 0.13 | *0.0088 |
f-I slope (Hz/nA) | 63 ± 4 | 80 ± 8 | 103 ± 16 | 0.12 | 0.19 | *0.0045 |
Ithresh (pA) | 267 ± 19 | 295 ± 26 | 163 ± 23 | 0.45 | *0.0030 | *0.0038 |
SFA ratio | 0.89 ± 0.02 | 0.89 ± 0.02 | 0.74 ± 0.05 | 0.91 | *0.0054 | *0.0080 |
Vthresh (mV) | −33 ± 1 | −35 ± 1 | −32 ± 1 | 0.19 | 0.21 | 0.85 |
AP amplitude (mV) | 72 ± 2 | 79 ± 2 | 66 ± 2 | *0.015 | *0.00078 | 0.028 |
AP width (msec) | 1.21 ± 0.08 | 0.98 ± 0.01 | 1.03 ± 0.02 | *0.0060 | 0.23 | 0.19 |
Vthresh − Vr (mV) | 46 ± 2 | 38 ± 2 | 37 ± 2 | * 0.00028 | 0.32 | * 0.0041 |
Values under each cell group are mean ± s.e.m. Numbers in the last three columns are p-values for comparisons between the indicated groups (rank-sum test; asterisks indicate significant differences). Vr, resting membrane potential; Rinput, input resistance; Cm, cell capacitance; Ithresh, current threshold for evoking action potential(s); SFA ratio, spike-frequency accommodation ratio; Vthresh, voltage threshold for action potential; AP amplitude, action potential peak minus threshold; AP width, action potential duration. See ‘Materials and methods’ for additional details.