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. 2015 Jan 12;5:777. doi: 10.3389/fpls.2014.00777

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Copyright © 2015 Manohar, Tian, Moreau, Park, Choi, Fei, Friso, Asif, Manosalva, von Dahl, Shi, Ma, Dinesh-Kumar, O'Doherty, Schroeder, van Wijk and Klessig.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immuno-blot analyses monitoring SA competition of 4AzSA crosslinking to recombinant cSABPs identified by the 4AzSA/immuno-selection screen. Photo-activated crosslinking of 50 ng/μl of the indicated recombinant purified proteins: (A) TRX-m1, (B) TPPII, (C) SHM4, (D) LOX2, and (E) ACX4 to 4AzSA (50 μM) in the absence or presence of increasing amounts of SA was detected by immuno-blotting using an α-SA antibody. Reactions without 4AzSA served as controls. Note that controls with 4AzSA but without photo-activation were previously shown to give similar results (Tian et al., 2012). Proteins stained with Coomassie brilliant blue (CBB) served as the loading control.