Skip to main content
. 2015 Jan 12;5:772. doi: 10.3389/fpls.2014.00772

Figure 4.

Figure 4

Molecular characterization of the GABI_418H02 pi3k mutant. (A) Schematic representation of the T-DNA insertion in PI3K gene (At1g60490). Boxes indicate exons. In GABI_418H02 mutant, T-DNA insertion is located in the fifth exon of the PI3K gene. Primer 1 (P1) corresponds to LB08409 primer specific to left border of the T-DNA and primers 2 (P2) and 3 (P3) are gene-specific primers used for the genotyping. (B) Expression analysis by RT-qPCR of AtPI3K, AtP5CS1 and AtProDH1 in WT and PI3K/pi3k hemizygous mutant. cDNA were obtained from 12-days-old WT and PI3K/pi3k seedlings. Results expressed as percentage compared to APT1 as a reference gene are means ± SD (n = 3). Significant differences of gene expression between WT and hemizygous seedlings are indicated by *(Student's T-Test, *P < 0.05 and **P < 0.01).