Table 1.
Strains and plasmids used in this study.
| Strains or plasmids | Genotype and relevant phenotype | References |
|---|---|---|
| STRAINS (CLASS) | ||
| E. coli (γ-Proteobacteria) | ||
| MG1685 (K12 Rif) | RifR mutant of MG1655 | Fox et al., 2008 |
| JM109 | endA1 recA1 gyrA96 thi hsdR17 (r−K m+K) relA1 supE44Δ (lac-proAB) [F′ traD36 proAB laqIqZ ΔM15] | Yanischperron et al., 1985 |
| DH5α | NalR | Sambrook et al., 1989 |
| S17-1 | SmR | Mazodier et al., 1989 |
| EC100 | SmR | Hale et al., 1984 |
| Agrobacterium tumefaciens C58 (α-Proteobacteria) | RifR | Wood et al., 2001 |
| Cupriavidus necator JMP 228 (β-Proteobacteria) | RifR | Top et al., 1995 |
| PLASMIDS | ||
| pBBR1MCS-5 | BHR mobilizable cloning vector; GmR | Yanischperron et al., 1985 |
| pB10 | Mercury-resistance | Schlüter et al., 2003 |
| pUTminiTn5Km1 | pUT replicon carrying miniTn5::Km1 | De Lorenzo et al., 1990 |