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. 2015 Jan 12;5:454. doi: 10.3389/fgene.2014.00454

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Copyright © 2015 Špírek, Poláková, Jatzová and Sulo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Origin of mitochondrial and nuclear genomes in cybrids. (A) HinfI digest of genomic DNA separated on 1% agarose gel. Lanes: S. cerevisiae MCC109 ρ⁰ (acceptor strain) (1); S. kudriavzevii CBS 8840 cybrid (2) parent (3); S. mikatae CBS 8839 cybrid (4) parent (5); S. cariocanus CBS 7994 cybrid (6) parent (7); S. paradoxus CBS 2908 cybrid (8) parent (9); S. paradoxus CBS 7400 cybrid (10) parent (11); S. paradoxus CBS 432 cybrid (12) parent (13); size standard λ/PstI (14). (B) HinfI digest of genomic DNA separated on 1% agarose gel. Lanes: S. bayanus CBS 380 cybrids (1–6) parent (7); size standard λ/PstI (8). (C) EcoRV digest of mtDNA separated on 1% agarose gel. Lanes: size standard λ/PstI (1, 14); S. kudriavzevii CBS 8840 cybrid (2) parent (3); S. mikatae CBS 8839 cybrid (4) parent (5); S. cariocanus CBS 7994 cybrid (6) parent (7); S. paradoxus CBS 2908 cybrid (8) parent (9); S. paradoxus CBS 7400 cybrid (10) parent (11); S. paradoxus CBS 432 cybrid (12) parent (13); size standard λ/PstI (14). (D) Pulse-field electrophoresis of cybrids (1–8) and parental species (9–16): Lanes 1–9 S. cerevisiae cybrids with mt genomes from S. bayanus CBS 380 (1); S. mikatae CBS 8839 (2); S. paradoxus CBS 7400 (3); S. paradoxus CBS 2908 (4); S. kudriavzevii CBS 8840 (5); S. cariocanus CBS 7994 (6); S. paradoxus CBS 432 (7); S. cerevisiae strain W303 1A (8); MCC109 ρ⁰ parental strain (9); S. paradoxus CBS 432 (10); S. cariocanus CBS 7994 (11); S. paradoxus CBS 2908 (12); S. kudriavzevii CBS 8840 (13); S. mikatae CBS 8839 (14) S. bayanus CBS 380 (15); S. cerevisiae strain W303 1A (16).