Figure 1.
Exposure to (R)-albuterol, but not (S)-albuterol or racemic (R + S)-albuterol, induces mRNA expression of 11β-HSD1, but not 11β-HSD2, in airway epithelial cells. MTCC were exposed to 10−6M (R)-albuterol (R), (S)-albuterol (S), racemic (R + S)-albuterol, or 10 ng/ml TNFα. Twenty-four hours later, RNA was isolated, cDNA was generated, and 11β-HSD1 (Hsd11b1), 11β-HSD2 (Hsd11b2), and GAPDH (Gapdh) gene expression were analyzed by quantitative PCR. Relative expression of 11β-HSD1 (A) and 11β-HSD2 (B) were calculated. Data were pooled from three separate experiments. n = 6–9/group; *p ≤ 0.05 and **p ≤ 0.01 compared to vehicle.