Figure 2.

Pre-exposure to (R)-albuterol reduces LPS- and TNFα-induced NF-κB activity in airway epithelial cells. NF-κB-luciferase MTCC were exposed to vehicle (veh.) or 10⁻⁶M (R)-albuterol (R), (S)-albuterol (S), or (R + S)-albuterol for 24 h. All cells except control were then exposed to 100 ng/ml LPS (A) or 10 ng/ml TNFα (B). Sixteen hours later, cell lysates were prepared, luciferase activity was measured, and protein concentration was determined. n = 5–6/group and the experiment was repeated twice; **p ≤ 0.01 compared to control, ***p ≤ 0.001 compared to control, ^#p ≤ 0.05 compared to vehicle, ^##p ≤ 0.01 compared to vehicle.