Figure 3.
Pre-exposure of airway epithelial cells to (R)-albuterol for 24 h, followed by cortisone exposure for 16 h, augments GRE-luciferase activity, which requires the activity of 11β-HSD. GRE-luciferase MTCC were exposed to 10−8M dexamethasone or 10−6M (R)-albuterol. Twenty-four hours later, 10−6M cortisone alone or with 10−6M glycyrrhetinic acid was added for 16 h and luciferase activity and total protein were measured. n = 5–6 samples/group and the experiment was repeated twice; ***p ≤ 0.001 compared to untreated, *p ≤ 0.05 compared to untreated, #p ≤ 0.05 compared to (R)-albuterol + cortisone.