Figure 4.
Pre-exposure of MTCC to (R)-albuterol and cortisone for 24 h, followed by exposure to LPS or TNFα for 16 h, diminishes NF-κB-luciferase activity, which requires the activity of 11β-HSD. NF-κB-luciferase MTCC were exposed for 24 h to 10−6M cortisone alone or to 10−6M (R)-albuterol with or without 10−6M cortisone and 10−6M glycyrrhetinic acid. Twenty-four hours later, 100 ng/ml LPS (A) or 10 ng/ml TNFα (B) were added to the cell culture medium. Cells were then lysed 16 h later using reporter lysis buffer. Luciferase activity and total protein were then measured. n = 4–6 samples/group and the experiment was repeated twice; ***p ≤ 0.001 compared to untreated, ###p ≤ 0.001 compared to LPS (A) or TNFα (B), #p ≤ 0.05 compared to LPS (A) or TNFα(B).