Table 3.

Data supporting the presence of a novel endothelial cannabinoid receptor

Read-out assay	Species and vascular bed	Agonist	pEC50	Reference
PTX-sensitive CB1/CB2 receptor-independent endothelium-dependent relaxation	RSMA	AEA	6.3 μM	White and Hiley, 1997
	RSMA	NAGly	5.8 μM	Parmarand Ho, 2010
	RSMA	ARA-S	4.9 μM	Parmar and Ho, 2010
	RA	AEA	5.9 μM	Herradon et al., 2007
	RA	THC	5.81 μM	O'Sullivan et al., 2005b
	RA	Abn-CBD	not presented	Milman et al., 2006
	RA	ARA-S	1–50 μM	Milman et al., 2006
	RMA	Abn-CBD	5.6 μM	Offertaler et al., 2003
	RabA	AEA	0.6 μM	Mukhopadhyay et al., 2002
PTX-sensitive activation of p42/44 MAPK	Cell type	Agonist	Concentrations	Reference
	HUVEC	Abn-CBD	30 μM	Mo et al., 2004
	HUVEC	Abn-CBD	10 μM	Offertaler et al., 2003;
	HEK293-GPR18, BV-2	NAGly	10 nM–10 μM	McHugh et al., 2010
	HEC-1B	NAGly, AEA	10 nM	McHugh et al., 2012
PTX-sensitive cell migration	Cell type	Agonist	Concentrations	Reference
	HUVEC	Abn-CBD	10–50 μM	Mo et al., 2004
	BV-2	NAGly	0.17 nM	McHugh et al., 2010
	BV-2	Abn-CBD	13.1 nM	McHugh et al., 2010
	HEK293-GPR18	NAGly	1 μM	McHugh et al., 2010
	HEK293-GPR18	Abn-CBD	1 μM	McHugh et al., 2010
Cell migration sensitive to PI3K/Akt inhibitors	Cell type	Agonist	Concentrations	Reference
	HUVEC	Abn-CBD	30 μM	Mo et al., 2004

Abn-CBD, abnormal cannabidiol; AEA, anandamide; ARA-S, N-arachidonyl serine; BV-2, mouse microglial cells; CB₁R-cannabinoid receptor type 1; CB₂R-cannabinoid receptor type 2; PTX, pertussis toxin; RA, rat aorta; RabA, rabbit aorta; RMA, rat mesenteric artery; RSMA, rat small mesenteric artery; THC, Δ⁹-tetrahydrocannabinol.