Figure 3.
Effect of M30 on HIF-1α and HIF-1α-related genes in the hippocampus of HFD mice. (A) A representative Western blot and analysis of mRNA expression levels of HIF-1α in the hippocampus of mice fed with ND or HFD treated with vehicle or M30 (5 mg⋅kg−1). Western blot graphs represent densitometry quantification of the lanes, normalized to β-actin. (B) mRNA expression levels of GLUT-1, GLUT-3, enolase-1, aldolase A, VEGF and HO-1. (C) mRNA expression levels of GLUT-1 and VEGF. The amount of each mRNA product was measured by quantitative real-time RT-PCR and normalized to the housekeeping gene γ-tubulin. Data are expressed as arbitrary units and represent means ± SEM (n = 7). #P < 0.05 versus ND mice; *P < 0.05 versus vehicle-treated HFD mice.