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. 2014 Dec 1;171(24):5650–5664. doi: 10.1111/bph.12868

Figure 1.

Figure 1

Subcellular distribution pattern of D1 and CRF2α receptors expressed in HEK293T cells. (A) Fluorescence of chimeric proteins. D1 receptorYFP and CRF2α receptorCFP expressed in HEK293T-cells, fluorescence images of YFP and CFP were obtained by confocal microscopy. The upper panel shows the surface fluorescence of D1 receptorYFP, and the bottom panel shows the intracellular fluorescence of CRF2α receptorCFP. (B) Immunofluorescence of wild-type receptors. D1 receptor (left) and CRF2α receptor (right) were detected by immnunofluorescence. Six fields of cells per independent experiment (n = 3) were examined. Individual cells were classified as having either fluorescence almost exclusively in a bright ring surrounding the cell (surface), or dense intracellular fluorescence (intracellular). Data are expressed as mean ± SEM and represent results from about 200 cells. *P ≤ 0.05, Mann–Whitney test. Scale bar: 10 μm. (C–D) Concentration–response curves showing cAMP levels. Cells expressing D1 receptors were stimulated with increasing concentration of SKF83959 (C); data are expressed as mean ± SEM (n = 3). Cells expressing CRF2α receptors were stimulated with increasing concentration of urocortin (UCN)I (D), data are expressed as mean ± SEM (n = 6).