Skip to main content
. 2014 Dec 1;171(24):5650–5664. doi: 10.1111/bph.12868

Figure 2.

Figure 2

D1 receptorYFP and CRF2α receptorCFP mutually modify their subcellular distribution when they are co-expressed. (A) Co-expression of chimeric protein D1 receptorYFP and CRF2α receptorCFP in HEK293T cells. Six fields of cells per independent experiment (n = 3) were examined. D1 receptorYFP acquires an intracellular phenotype in cells co-expressing CRF2α receptorCFP, with high degree of co-distribution in the merge. The cells that only expressed D1 receptorYFP maintained their surface phenotype (asterisk). (B) Quantification of surface and intracellular phenotypes when D1 receptorYFP is expressed alone or with CRF2α receptorCFP. The percentage of cells with receptors at the cell surface is greater when D1 receptorYFP was expressed alone, lower when CRF2α receptorCFP was expressed alone, and an intermediate level was obtained when the receptors where co-expressed. Data are expressed as mean ± SEM and represent results from about 200 cells of three independent experiments (n = 3) *P ≤ 0.05, Mann–Whitney test. (C) Co-expression of CRF1 receptorYFP and CRF2α receptorCFP. The subcellular localization of both receptors is maintained.