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Concentration–response curves for cAMP accumulation. HEK293T cells expressing D1 receptors and CRF2α receptor alone and cells co-expressing D1 plus CRF2α receptors were incubated in the presence of different concentrations of SKF83959 (A) or urocortin (UCN) I (B) with or without 10 μM SCH23390 (SCH). The results are the mean ± SEM of three independent experiments.
