Figure 7.
Calcium mobilization induced by the activation of D1 receptors. HEK293T cells expressing D1 receptors alone or D1 plus CRF2α receptors were loaded with FURA-2 AM. Intracellular calcium mobilization was measured in living cells at 340 and 380 nm in a confocal microscope and time lapse of the 340/380 ratio was plotted. The selective D1 receptor agonist, SKF83959, generated calcium mobilization when the receptors were co-expressed (n = 71), but no effect was observed when D1 receptors were expressed alone (n = 67). The data were obtained from four independent experiments.