. 2014 Dec 1;171(24):5650–5664. doi: 10.1111/bph.12868

Table 1.

Constructs information

Construct	Vector	Restriction enzyme	Primers
D₁ receptor	pcDNA 3.1	EcoRV and NotI	CCGGATATCGGCGCCAACATGAGGACTCTGAACACC
D₁ receptor	pcDNA 3.1	EcoRV and NotI	CGAGCGGCCGCTCAGGTTGGGTGCTGACCGTT
CRF_2α receptor	pcDNA 3.1	HindIII and XhoI	CCGAAGCTTGGCGCCACCATGGACGCGGCACTGCTC
CRF_2α receptor	pcDNA 3.1	HindIII and XhoI	CGACTCGAGTCACACAGCGGCCGTCTGCTT
D₁ receptor^YFP	pEYFP-N1	EcoRI and BamHI	CTCGAATTCGCCACCATGAGGACTCTGAACACC
D₁ receptor^YFP	pEYFP-N1	EcoRI and BamHI	CGTCGCCGTCCAGCTCGACCAG
CRF_2α receptor^CFP	pECFP-N1	EcoRI and KpnI	CCGGAATTCGCCACCATGGACGCGGCACTG
CRF_2α receptor^CFP	pECFP-N1	EcoRI and KpnI	CGTCGCCGTCCAGCTCGACCAG
CRF₁ receptor^YFP	pEYFP-N1	EcoRI and KpnI	GTGGAATTCACCATGGGAGGGCAC
CRF₁ receptor^YFP	pEYFP-N1	EcoRI and KpnI	CCGCGGTACCCAGACTGCTGTGGA
D₁ receptor^myc/His	pcDNA 3.1 myc/His	EcoRV and BamHI	CCGGATATCGGCGCCAACATGAGGACTCTGAACACC
D₁ receptor^myc/His	pcDNA 3.1 myc/His	EcoRV and BamHI	CGAGGATCCGGTTGGGTGCTGACCGTT
CRF_2α receptor^R^luc	pRluc-N1	EcoRI and KpnI	CTCGAATTCGCCACCATGGACGCGGCACTGCTC CGCGGTACCGCCACAGCGGCCGTCTGCTTG
D₁ receptor-NLS^YFP	pEYFP-N1	EcoRI and BamHI	CTCGAATTCGCCACCATGAGGACTCTGAACACC CCTAAGAGGGTTGAAAATCTTTTAAATTTTTTAGCATTAAAGGCATAAATG GCCTTTAATGCTAAAAAATTTAAAAGATTTTCAACCCTCTTAGGATGC CGTCGCCGTCCAGCTCGACCAG
CRF_2α receptor^Flag	pcDNA 3.1	HindIII and NotI	CCGAAGCTTGGCGCCACCATGGACGCGGCACTGCTC GTCGTCATCCTTGTAGTCCACAGCGGCCGTCTGCTT
CRF_2α receptor^Flag	pcDNA 3.1	HindIII and NotI	GACTACAAGGATGACGACGATAAGTGAGCG GAATAGAATGACACCTACTCAGACAATCGC

Each construct was generated by PCR amplification and the primers used are listed. The restriction enzymes and vectors into which they were sub-cloned are also indicated.