Basal superoxide production in corpus cavernosum tissue from LZR and OZR detected by lucigenin-enhanced chemiluminescence. Effect of ET-1 (10 nM) on basal superoxide production, and of tempol (100 μM) on the ET-1-induced superoxide production, in the absence and presence of the NOS inhibitor L-NOARG (100 μM). Superoxide production is expressed in counts per minute (cpm) mg−1 of tissue. Data are shown as the means ± SEM of n = 4 animals (two corpus cavernosum samples from each animal). *P < 0.05, **P < 0.01 versus basal, †P < 0.05 versus ET-1-treated, #P < 0.05 versus LZR. Student's t-test for unpaired observations.