Figure 3.

Basal superoxide production in corpus cavernosum tissue from LZR and OZR detected by lucigenin-enhanced chemiluminescence. Effect of the inhibitors of NADPH oxidase, apocynin (30 μM) and diphenylene iodonium (DPI) (10 μM) on both basal and ET-1 (10 nM)-stimulated superoxide production, and of the activator of PKC PDBu (10 μM) on the basal superoxide generation in corpus cavernosum tissue from LZR and OZR detected by lucigenin-enhanced chemiluminescence and expressed in counts per minute (cpm) mg⁻¹ of tissue. Experiments were performed in the presence of the NOS inhibitor L-NOARG (100 μM). Data are shown as the means ± SEM of n = 3–4 animals (two corpus cavernosum samples from each animal). *P < 0.05 versus basal, ^†P < 0.05 versus ET-1-treated, ^#P < 0.05 versus LZR; Student's t-test for unpaired observations.