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Constitutive activity of GPR119. (A) Surface expression of FLAG-tagged GPR119 resulting from transfections with increasing concentrations of a GPR119 construct and measured with elisa. (B) Basal level and AR231453-induced cAMP accumulation from cells transfected with increasing concentrations of the GPR119 construct – 20 μg of DNA was chosen as the optimal amount for further studies.
