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. 2014 Dec 20;14:299. doi: 10.1186/s12866-014-0299-y

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© Romão et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The aggregative adherence-like pattern shown by aEPEC 1551–2 at high glucose concentration is dependent on type 1 pili production. (A) Relative expression of fimA in presence of 5 mM (calibrator) and 25 mM glucose at 1.5 h, 3 h and 6 h. The data showed that at 3 and 6 h, there was an increase in fimA expression of 75 ± 21% and 103 ± 15%, respectively. (B, C, D and E) Adherence patterns observed with the wild-type strain (B), 1551-2ΔfimA (C), 1551-2ΔfimA (pBAD) (D) and 1551-2ΔfimA (pFimA) (E). The 1551-2ΔfimA mutant maintained the LA pattern on HeLa cells, but the AA-like pattern was no longer observed, pointing to the involvement of T1P in the establishment of this phenotype. * and ** indicate statistical differences observed between experiments performed at 5 and 25 mM glucose after 3 h (P =0.02) and 6 h (P =0.002) of incubation, respectively.