Table 1.
Bacterial strains and plasmids used in this study
| E. coli strains and plasmids | Characteristics | Reference |
|---|---|---|
| Strains | ||
| 1551-2 | aEPEC (ONT:H−) isolated from a diarrheic child, which expresses LA on HeLa cells (NalR) | [18] |
| 1551-2ΔfimA | 1551-2 fimA::zeo (NalR ZeoR), T1P mutant | [24] |
| 1551-2ΔfimA(pBAD) | 1551-2 mutant harboring pBAD/Myc-His A vector (NalR ZeoR AmpR) | [24] |
| 1551-2ΔfimA(pFimA) | 1551-2 mutant harboring pFimA (NalR ZeoR AmpR) | [24] |
| Plasmid | ||
| pBAD/Myc-His A | Cloning vector, harboring the araBAD promoter (pBAD) from E. coli, which is induced in the presence of L-arabinose. | Invitrogen |
| pFimA | pBAD/Myc-His A vector harboring the fimA gene from aEPEC 1551-2 | [24] |
NalR: nalidixic acid resistant; ZeoR: zeocin resistant; AmpR: ampicillin resistant; LA: Localized adherence.