(A and B) The percentages of eliminated (gray bars) and added (black bars) spines in
1-month-old (A) and 5-month-old (B) mice injected with saline (con) or bicuculline (bic)
solution. Statistical analysis was performed using One-Way ANOVA with the Holm-Sidak
method for pair-wise multiple comparison. Data are presented as the mean ±
s.e.m.
(C) Spine dynamics in animals that received daily injections of saline (con) or
bicuculline (bic) prior to the final imaging session are shown. In addition, spine
dynamics in animals subjected to the withdrawal experimental paradigm (denoted with
“withdrawal”) are shown. For these animals, daily injections of saline
(con) and bicuculline (bic) were administered for the first 7 days followed by no
injections for the next 7 days prior to the final view. Statistical analysis was performed
using One-Way ANOVA with the Holm-Sidak method for pair-wise multiple comparison. Data are
presented as the mean ± s.e.m.
(D) Survival fractions of spines in saline- (con) and bicuculline-treated (bic) mice. The
box plot shows the extreme values, the 25% and 75% percentiles, medians
and error bars. The mean lines are displayed in the plot in red. Statistical analysis was
performed using Student’s one-tailed unpaired t-test.