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. Author manuscript; available in PMC: 2015 Dec 20.
Published in final edited form as: FEBS Lett. 2014 Nov 4;588(24):4551–4560. doi: 10.1016/j.febslet.2014.10.023

Figure 3. Bicuculline blockade of GABA-A receptors enhances spine elimination without affecting spine formation.

Figure 3

(A and B) The percentages of eliminated (gray bars) and added (black bars) spines in 1-month-old (A) and 5-month-old (B) mice injected with saline (con) or bicuculline (bic) solution. Statistical analysis was performed using One-Way ANOVA with the Holm-Sidak method for pair-wise multiple comparison. Data are presented as the mean ± s.e.m.

(C) Spine dynamics in animals that received daily injections of saline (con) or bicuculline (bic) prior to the final imaging session are shown. In addition, spine dynamics in animals subjected to the withdrawal experimental paradigm (denoted with “withdrawal”) are shown. For these animals, daily injections of saline (con) and bicuculline (bic) were administered for the first 7 days followed by no injections for the next 7 days prior to the final view. Statistical analysis was performed using One-Way ANOVA with the Holm-Sidak method for pair-wise multiple comparison. Data are presented as the mean ± s.e.m.

(D) Survival fractions of spines in saline- (con) and bicuculline-treated (bic) mice. The box plot shows the extreme values, the 25% and 75% percentiles, medians and error bars. The mean lines are displayed in the plot in red. Statistical analysis was performed using Student’s one-tailed unpaired t-test.