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. Author manuscript; available in PMC: 2015 Jan 12.
Published in final edited form as: Toxicol Sci. 2004 Jun 30;81(2):467–479. doi: 10.1093/toxsci/kfh205

FIG. 4.

FIG. 4

Effect of several soluble metal salts on IL-6 release in the presence and absence of MALP-2. A composite cell line representing early passage-cells pooled from six different donors was seeded into 24-well plates and allowed to attach for 48 h and then exposed to varying concentrations of the specific metal salts in the presence or absence of MALP-2 (600 pg/ml) in serum-free MEM with 0.1% BSA for 48 h. IL-6 was measured in the conditioned media by ELISA and normalized to DNA content of the monolayer at the end of the incubation. Data represent mean ± SEM of four different experiments. *Denotes statistically significant difference compared to control cells in the absence of any stimuli, and **denotes statistically significant difference compared to cells treated with MALP-2 alone by one-way ANOVA and Dunnett’s multiple comparison to control.