TABLE 4.

Sensitivity, specificity, and confidence interval for the subset of tests performed by USPHLs in all diagnostic categories, by DNA extraction method

Organism	DNA extraction method	na	Sensitivity (% [95% CI])	Specificity (% [95% CI])
B. pertussis	All	907	96.1 (93.2–97.9)	95.2 (93.2–96.8)
	Automated, EasyMAG	062	100 (83.2–100)	100 (91.6–100)
	Automated, MagNA Pure	371	94.3 (88.6–97.7)	95.6 (92.2–97.8)
	Crude boil, heat lysis	84	100 (87.7–100)	100 (93.6–100)
	Spin column, Qiagen	270	94.6 (87.9–98.2)	94.9 (90.6–97.6)
	Not specified	120	100 (91.2–100)	88.8 (79.7–94.7)
B. parapertussis	All	844	98.6 (95.9–99.7)	99.7 (98.9–100)
	Automated, EasyMAG	77	100 (83.9–100)	100 (93.6–100)
	Automated, MagNA Pure	348	98.8 (93.8–100)	100.0 (98.6–100)
	Crude boil, heat lysis	84	95.2 (76.2–99.9)	98.4 (91.5–100)
	Spin column, Qiagen	286	98.6 (92.5–100)	99.5 (97.4–100)
	Not specified	49	100 (73.5–100)	100 (90.5–100)
B. holmesii	All	666	98.2 (94.7–99.6)	97.8 (96.1–98.9)
	Automated, EasyMAG	60	100 (78.2–100)	93.3 (81.7–98.6)
	Automated, MagNA Pure	311	97.4 (91.0–99.7)	99.6 (97.6–100)
	Crude boil, heat lysis	72	94.4 (72.7–99.9)	96.3 (87.3–99.5)
	Spin column, Qiagen	175	100 (91.4–100)	97.0 (92.5–99.2)
	Not specified	48	100 (73.5–100)	97.2 (85.5–99.9)
B. pertussis/B. holmesii (undifferentiated)	All	330	97.6 (94.4–99.2)	96.0 (90.9–98.7)
	Automated, EasyMAG	19	100 (76.8–100)	100 (47.8–100)
	Automated, MagNA Pure	144	94.4 (87.4–98.2)	90.9 (80.0–97.0)
	Crude boil, heat lysis	72	100 (91.6–100)	100 (88.4–100)
	Spin column, Qiagen	92	100 (93.7–100)	100 (90.0–100)
	Not specified	3	100 (29.2–100)

^an, number of tests reported for each organism, including tests of positive and negative samples.